癌胚抗原相关细胞粘附分子3(CEACAM3)重组蛋白说明书

CEACAM3  Recombinant protein  

[ DESCRIPTION ]

 Protein Names: Carcinoembryonic Antigen Related Cell Adhesion Molecule 3

 Synonyms:CEACAM3, Cea1

Species: Rat

Size: 100µg

Source:Escherichia coli-derived

[ PROPERTIES ]

Residues: Ser273~Leu416 (Accession # Q63111), with N-terminal His-Tag.

Grade & Purity: >95%, 17 kDa as determined by SDS-PAGE reducing conditions.

 Formulation: Supplied as liquid form in Phosphate buffered saline(PBS), pH 7.4.

 Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method).

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Molecular Mass: 17.1 kDa

Predicted isoelectric point: 7.2

[ PREPARATION ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8ºC for one month.

Aliquot and store at -80ºC for 12 months.

Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate wasdetermined by accelerated thermal degradation test, that is, incubate the protein at 37ºC for 48h, and no obviousdegradation and precipitation were observed. (Referring from China Biological Products Standard, which wascalculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date underappropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-tag, its sequence is listed below.

MGHHHHHHSG SEF-SAQLTVE SAPTSVAEGA SVLLLVHNLP ENLRAIFWYK GVILFKDLEV ARYVIGTNSS VLGPAHSGRETMYSNGSLLL QNVTRNDAGF YTLRTLSTDL KAKVVHVQLQ VNTSSCCDPL TPALLTIDPV PRHAAKGESV LLQVRNL

[ REFERENCES ]

1. Rebstock S., et al. (1990) J. Biol. Chem. 265:7872-7879.
2. Kodelja V., et al. (1989) J. Biol. Chem. 264:6906-6912.
3. Kwitek A.E., et al. (2004) Genome Res. 14:750-757.
4. Khan WN., et al. (1992) Genomics 14 (2): 384–90.