Cyclic nucleotide ELISA kits tailored to provide highly sensitive results for accurate determination of intracellular and extracellular cAMP/cGMP levels。

Flexible Sample Preparation

Cayman Chemical’s Cyclic AMP (cAMP) and Cyclic GMP (cGMP) ELISA Kits are competitive assays that can detect sub-picomolar concentrations of cAMP/cGMP in various sample types. Acetic anhydride is provided in Item Nos.

581001 and 581021 for use in an optional acetylation procedure prior to sample analysis. Because acetylation yields up to a 10-fold increase in assay sensitivity it is often recommended, but depending on your research application, this initial sample preparation step is not always necessary.* Use the comparison chart below of typical assay ranges to determine if your sample would benefit from a pre-assay acetylation step. Note that Cayman’s Cyclic AMP Select ELISA Kit achieves excellent sensitivity without the need to perform a pre-assay acetylation step.

Cayman’s AChE Tracer Advantage

Cayman’s cAMP/cGMP ELISAs were designed with competitive assay principles and employ AChE as the

detection enzyme. AChE offers these unique advantages:

· Fast turnover rate (3X faster than HRP or AP)

· Active in a wide pH range (pH 5.0-10.0)

· Does not self-inactivate during turnover

· Enables redevelopment if assay is accidentally splashed or spilled

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