PRMT5 (C-term) Peptide
产品名称： PRMT5 (C-term) Peptide
英文名称： PRMT5 (C-term) Peptide
产品编号： P0148
产品价格： 0
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围： null
亚诺法生技股份有限公司（Abnova)
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- 邮编 : 11493
- 所在区域 : 台湾
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- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of PRMT5.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human PRMT5.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- mSin3A/histone deacetylase 2- and PRMT5-containing Brg1 complex is involved in transcriptional repression of the Myc target gene cad.
Pal S, Yun R, Datta A, Lacomis L, Erdjument-Bromage H, Kumar J, Tempst P, Sif S.Mol Cell Biol. 2003 Nov;23(21):7475-87.
- 2.
- Prmt5, which forms distinct homo-oligomers, is a member of the protein-arginine methyltransferase family.
Rho J, Choi S, Seong YR, Cho WK, Kim SH, Im DS.J Biol Chem. 2001 Apr 6;276(14):11393-401. Epub 2001 Jan 10.
- 3.
- The human homologue of the yeast proteins Skb1 and Hsl7p interacts with Jak kinases and contains protein methyltransferase activity.
Pollack BP, Kotenko SV, He W, Izotova LS, Barnoski BL, Pestka S.J Biol Chem. 1999 Oct 29;274(44):31531-42.
- Applications
- Western Blot (Cell lysate)
- The PRMT5 polyclonal antibody (Cat # PAB2175) is used in Western blot to detect PRMT5 in HL-60 cell lysate.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.