Description

Invent Biotechnologies MinuteTM single cell isolation kit is composed of optimized tissue
disaggregation buffers and specially designed filter cartridges with 2.0 ml collection tubes. The
kit is designed to rapidly isolate single cells/nuclei from fresh/frozen/formaldehyde fixed animal
tissues. The tissue disaggregation buffers are formulated to gently disaggregate animal tissues.
The buffers don’t contain any proteinases that may have adverse effects on cell surface marker
detection. Due to the use of filter cartridges with pre-defined pore size and thickness, single cell
suspension can be isolated from fresh or formaldehyde fixed tissues in less than 8 min with high
yield.
Application
Single cells isolated with this kit can be used as starting materials for FACS and chromosome
immunoprecipitation (ChIP) analysis. The single cell suspension can also be used as a starting
material for isolation/purification of DNA, RNA, proteins and other cellular components.
Buffer Formulation: Proprietary
Kit components
1. 25 ml buffer A (for non-fixed tissues)
2. 25 ml buffer B (for formaldehyde fixed tissues)
3. 50 protein extraction filter cartridges
4. 50 collection tubes with cap
5. Plastic rod (4)
Storage: Store the kit at 4OC
Additional Materials Required
Table-Top Microcentrifuge
1 X PBS or FACS buffer (1 X PBS with 5% FBS or BSA)