MTOR polyclonal antibody
产品名称： MTOR polyclonal antibody
英文名称： MTOR polyclonal antibody
产品编号： PAB12715
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of MTOR.
- Immunogen:
- A synthetic peptide corresponding to C-terminus of human MTOR.
- Host:
- Rabbit
- Theoretical MW (kDa):
- 290
- Reactivity:
- Bovine, Chicken, Dog, Human, Mouse, Rat
- Specificity:
- This antibody only recognizes ~290 KDa of human MTOR.
- Form:
- Liquid
- Storage Buffer:
- In PBS, pH 7.2 (10% Proclin300)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
The optimal working dilution should be determined by the end user.
- Publication Reference
- 1.
- Dissociation of raptor from mTOR is a mechanism of rapamycin-induced inhibition of mTOR function.
Oshiro N, Yoshino K, Hidayat S, Tokunaga C, Hara K, Eguchi S, Avruch J, Yonezawa K.Genes Cells. 2004 Apr;9(4):359-66.
- Applications
- Immunoprecipitation
- The cell lysate derived from MCF-7 was immuno-precipitated by MTOR polyclonal antibody (Cat # PAB12715), resolved onto 7.5% SDS-PAGE, transferred onto NC membrane, then immuno-blotted by MTOR polyclonal antibody (Cat # PAB12715) at 1 : 500.
An immunoreactive band around ~290 kDa was observed (lane 1). Lane 2 is a negative control.
- Entrez GeneID:
- 2475
- Gene Name:
- MTOR
- Gene Alias:
- FRAP,FRAP1,FRAP2,RAFT1,RAPT1
- Gene Description:
- mechanistic target of rapamycin
- Omim ID:
- 601231
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq
- Other Designations:
- FK506 binding protein 12-rapamycin associated protein 1,FK506 binding protein 12-rapamycin associated protein 2,FK506-binding protein 12-rapamycin complex-associated protein 1,FKBP-rapamycin associated protein,FKBP12-rapamycin complex-associated protein 1