Description
Invent Biotechnologies MinuteTM bacterial total protein extraction kit is composed of
optimized denaturing cell lysis buffers and protein extraction filter cartridges with 2.0 ml
collection tubes. The kit is designed to rapidly extract denatured proteins from bacteria.
The kit contains sufficient materials for extraction of total proteins from 100 ml
E.coli culture.
Application
MinuteTM bacterial total protein extraction kit is designed to rapidly extract total proteins
from cultured bacterial cells for applications such as SDS-PAGE, immunoblottings,
ELISA and other applications. This kit provides the most rapid method for preparation of
whole bacterial cell extract.
Buffer Formulation: Proprietary
Kit Components
1. 25 ml bacterial lysis buffer A (Part#: SB-004-A)
2. 2.5 ml buffer B (Part#: SB-004-B)
3. 50 protein extraction filter cartridges (Part#: P-001)
4 50 collection tubes with cap (Part#: P-002)
Storage
Store at room temperature.
Important Product Information
The MinuteTM bacterial total protein extraction kit is designed to extract total protein
rapidly from bacteria. The use of protease inhibitors is not necessary prior to extraction.
However if downstream application takes significant amounts of time or the protein
extract will be stored for longer period of time, addition of protease inhibitor to extracted
lysate is recommended. For determination of protein concentration BCA kit (Pierce) is
recommended. The protein extraction filter cartridge has the capacity of 500 µl. Multiple
filter cartridges may be used if larger amount of cell lysate is desired. To study protein
phosphorylation, phosphatase inhibitors must be added to lysis buffer prior to use.