产品概述：Purified recombinant protein of Human cleavage and polyadenylation specific factor 4, 30kDa (CPSF4), transcript variant 2, full length, with N-terminal GST and C-terminal His tag, expressed in E. coli, 50ug

蛋白长度：A DNA sequence encoding human full-length CPSF4

表达系统：E. coli

种属：Human

标签类型：N-GST and C-His

分子量：53.4 kDa

通用名：CPSF4

总结：Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs. Multiple alternatively spliced transcript variants that encode different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

形式：Liquid

浓度：>50 ug/mL as determined by microplate BCA method

纯度：> 80% as determined by SDS-PAGE and Coomassie blue staining

储存溶液：25mM Tris, pH8.0, 150mM NaCl, 10% glycerol, 1% Sarkosyl.

别名：CPSF30; NAR; NEB-1; NEB1