PTEN (phospho S385) polyclonal antibody
产品名称： PTEN (phospho S385) polyclonal antibody
英文名称： PTEN (phospho S385) polyclonal antibody
产品编号： PAB12751
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of PTEN.
- Immunogen:
- Synthetic phosphopeptide corresponding to residues surrounding S385 of human, mouse and rat PTEN.
- Sequence:
- DTSDP
- Host:
- Rabbit
- Reactivity:
- Bovine, Chicken, Human, Mouse, Rat
- Specificity:
- This antibody recognizes PTEN with a phosphorylated site at Serine 385. It does not cross-react with non-phosphospecific peptide.
- Form:
- Liquid
- Storage Buffer:
- In TBS, pH 7.2 (10% Proclin300)
- Storage Instruction:
- Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (1:200)
The optimal working dilution should be determined by the end user.
- Publication Reference
- 1.
- Cooperative phosphorylation of the tumor suppressor phosphatase and tensin homologue (PTEN) by casein kinases and glycogen synthase kinase 3beta.
Al-Khouri AM, Ma Y, Togo SH, Williams S, Mustelin T.J Biol Chem. 2005 Oct 21;280(42):35195-202. Epub 2005 Aug 17.
- Applications
- Western Blot (Tissue lysate)
- The mouse brain tissue lysate resolved onto 10% SDS-PAGE, transferred onto NC membrane, and followed by an immunoblotting with PTEN (phospho S385) polyclonal antibody (Cat # PAB12751, lane 1) at 1 : 500, or with a pre-incubation of immunizing peptide (lane 2).
- Dot Blot (Peptide)
- Dot Blot : 1 ug peptide was blot onto NC membrane.
A : PTEN (pS385, Phospho-specific).
B : PTEN (Paired S385, Non-phospho-speicfic).
C : Non-related phospho-specific peptide.
Followed were blotted at a 1 : 2000 dilution by :
1. PTEN (phospho S385) polyclonal antibody (Cat # PAB12751).
2. PTEN polyclonal antibody (Cat # PAB12725).